RAIDR– A Rapid Method for the Microextraction of O-Glycans

Technology #d-1341

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Researchers
Yehia Mechref
Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas
External Link (www.depts.ttu.edu)
Ahmed Abdelmonem
Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas
Lucas Veillon
Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas
Byeong Cho
Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas
Managed By
Cameron Smith
Licensing Associate 806-834-6822
Patent Protection

Provisional Patent Application Filed

O-glycosylation, a common post-translational modification of serine and threonine residues, regulates biological processes through the direct interactions of glycans with binding proteins. O-glycosylation modulates protein stability, conformation, solubility, and lifespan; however, it has also been implicated in the pathogenesis of a number of diseases, which has stimulated interest in analytical methods capable of isolating O-glycans for further characterization.  

The technology is a method termed Rapid Ammonium hydroxide Isobutyric acid O-glycan Deglycosylation Reaction (RAIDR) for the microscale extraction of O-glycans for glycomic characterization. When carried out with the assistance of microwave radiation, efficient O-glycan release can be achieved in 15 minutes; this is the most rapid method of releasing O-linked oligosaccharide reported to date. The RAIDR method also allows for extreme selectivity, resulting in almost exclusive liberation of O-glycans with minimal N-glycans released. Tests have indicated that less than 1% of total N-glycans were liberated during RAIDR release, demonstrating the method's high selectivity for O-glycans. Experiments involving the analysis of human blood serum support the conclusion that the RAIDR protocol represents a rapid, inexpensive, and selective method for the microextraction of O-glycans.  

Reference Number: D-1341 

Market Applications:

  • Pharmaceutical science
  • Glycomics research 

Features, Benefits, & Advantages: 

  • Fastest method known for O-linked oligosaccharides release from glycoconjugates
  • Inexpensive reagents
  • Selective for O-glycans 

Intellectual Property: 

  • A US. provisional patent, serial number 62423567, was filed on 11/17/16. 

Development Stage: 

  • The invention has been produced and tested.  

Researchers:

  • Yehia Mechref, Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas 
  • Ahmed Abdelmonem, Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas
  • Lucas Veillon, Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas
  • Byeong Cho, Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas 

Keywords: deglycosylation, glycoprotein, O-glycosylation, glycan, glycoconjugate